• Immunofluorescence analysis of L929 cells using NDUFB7 Rabbit polyclonal antibody (STJ115648) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of NDUFB7 in paraffin-embedded rat heart using NDUFB7 Rabbit polyclonal antibody (STJ115648) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of NDUFB7 in paraffin-embedded mouse liver using NDUFB7 Rabbit polyclonal antibody (STJ115648) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of NDUFB7 in paraffin-embedded human liver using NDUFB7 Rabbit polyclonal antibody (STJ115648) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using NDUFB7 Rabbit polyclonal antibody (STJ115648) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NDUFB7 antibody (1-137) (STJ115648)

SKU:
STJ115648

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDUFB7 (1-137) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFB7
Gene ID: 4713
Uniprot ID: NDUB7_HUMAN
Immunogen Region: 1-137
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-137 of human NDUFB7 (NP_004137.2).
Immunogen Sequence: MGAHLVRRYLGDASVEPDPL QMPTFPPDYGFPERKEREMV ATQQEMMDAQLRLQLRDYCA HHLIRLLKCKRDSFPNFLAC KQERHDWDYCEHRDYVMRMK EFERERRLLQRKKRREKKAA ELAKGQGPGEVDPKVAL
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protein Name Nadh Dehydrogenase Ubiquinone 1 Beta Subcomplex Subunit 7
Cell Adhesion Protein Sqm1
Complex I-B18
Ci-B18
Nadh-Ubiquinone Oxidoreductase B18 Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Mitochondrion Intermembrane Space
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone 1 Beta Subcomplex Subunit 7 antibody
Anti-Cell Adhesion Protein Sqm1 antibody
Anti-Complex I-B18 antibody
Anti-Ci-B18 antibody
Anti-Nadh-Ubiquinone Oxidoreductase B18 Subunit antibody
Anti-NDUFB7 antibody

Information sourced from Uniprot.org

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