• Immunofluorescence analysis of L929 cells using NDUFB1 Rabbit polyclonal antibody (STJ11101440) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using NDUFB1 Rabbit polyclonal antibody (STJ11101440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NDUFB1 antibody (1-105) (STJ11101440)

SKU:
STJ11101440

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDUFB1 (1-105) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFB1
Gene ID: 4707
Uniprot ID: NDUB1_HUMAN
Immunogen Region: 1-105
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human NDUFB1 (NP_004536.2).
Immunogen Sequence: MICWRHPSAPCGRGEWQVPR SQLPLARVEFPVALGLGVAV GAEAAAIMVNLLQIVRDHWV HVLVPMGFVIGCYLDRKSDE RLTAFRNKSMLFKRELQPSE EVTWK
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protein Name Nadh Dehydrogenase Ubiquinone 1 Beta Subcomplex Subunit 1
Complex I-Mnll
Ci-Mnll
Nadh-Ubiquinone Oxidoreductase Mnll Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone 1 Beta Subcomplex Subunit 1 antibody
Anti-Complex I-Mnll antibody
Anti-Ci-Mnll antibody
Anti-Nadh-Ubiquinone Oxidoreductase Mnll Subunit antibody
Anti-NDUFB1 antibody

Information sourced from Uniprot.org

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