• Immunofluorescence analysis of HeLa cells using NDUFAF1 Rabbit monoclonal antibody (STJ11103545) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using NDUFAF1 Rabbit monoclonal antibody (STJ11103545) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NDUFAF1 antibody (1-150) [S5MR] (STJ11103545)

SKU:
STJ11103545

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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-NDUFAF1 (1-150) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFAF1
Gene ID: 51103
Uniprot ID: CIA30_HUMAN
Immunogen Region: 1-150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human NDUFAF1 (Q9Y375).
Immunogen Sequence: MALVHKLLRGTYFLRKFSKP TSALYPFLGIRFAEYSSSLQ KPVASPGKASSQRKTEGDLQ GDHQKEVALDITSSEEKPDV SFDKAIRDEAIYHFRLLKDE IVDHWRGPEGHPLHEVLLEQ AKVVWQFRGKEDLDKWTVTS DKTIGGRSEV
Tissue Specificity Ubiquitous.
Function As part of the MCIA complex, involved in the assembly of the mitochondrial complex I.
Protein Name Complex I Intermediate-Associated Protein 30 - Mitochondrial
Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Assembly Factor 1
Database Links Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion
Mitochondrion Matrix
Peripherally Associated With The Matrix Face Of The Mitochondrial Inner Membrane
Alternative Antibody Names Anti-Complex I Intermediate-Associated Protein 30 - Mitochondrial antibody
Anti-Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Assembly Factor 1 antibody
Anti-NDUFAF1 antibody
Anti-CIA30 antibody
Anti-CGI-65 antibody

Information sourced from Uniprot.org

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