• Western blot analysis of Mouse heart, using NDUFA9 Rabbit polyclonal antibody (STJ24719) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-NDUFA9 antibody (1-270) (STJ24719)

SKU:
STJ24719

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDUFA9 (1-270) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFA9
Gene ID: 4704
Uniprot ID: NDUA9_HUMAN
Immunogen Region: 1-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human NDUFA9 (NP_004993.1).
Immunogen Sequence: MAAAAQSRVVRVLSMSRSAI TAIATSVCHGPPCRQLHHAL MPHGKGGRSSVSGIVATVFG ATGFLGRYVVNHLGRMGSQV IIPYRCDKYDIMHLRPMGDL GQLLFLEWDARDKDSIRRVV QHSNVVINLIGRDWETKNFD FEDVFVKIPQAIAQLSKEAG VEKFIHVSHLNANIKSSSRY LRNKAVGEKVVRDAFPEAII VKPSDIFGREDRFLNSFASM HRFGPIPLGSLGWKTVKQP
Post Translational Modifications Acetylated on lysine residues. BLOC1S1 is required for acetylation. Acetylated by CLOCK in a circadian manner.
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Required for proper complex I assembly. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protein Name Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Subunit 9 - Mitochondrial
Complex I-39kd
Ci-39kd
Nadh-Ubiquinone Oxidoreductase 39 Kda Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Subunit 9 - Mitochondrial antibody
Anti-Complex I-39kd antibody
Anti-Ci-39kd antibody
Anti-Nadh-Ubiquinone Oxidoreductase 39 Kda Subunit antibody
Anti-NDUFA9 antibody
Anti-NDUFS2L antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance