![Western blot analysis of various lysates using [KO Validated] NDUFA7 Rabbit pAb (STJ110739) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/88942/405386/STJ110739_1__25973.1744891138.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] NDUFA7 Rabbit pAb (STJ110739) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.")
![Western blot analysis of lysates from wild type (WT) and NDUFA7 knockout (KO) 293T cells, using [KO Validated] NDUFA7 Rabbit pAb (STJ110739) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/88942/405387/STJ110739_2__66293.1744891139.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NDUFA7 knockout (KO) 293T cells, using [KO Validated] NDUFA7 Rabbit pAb (STJ110739) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.")
![Western blot analysis of various lysates using [KO Validated] NDUFA7 Rabbit pAb (STJ110739) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/88942/405386/STJ110739_1__25973.1744891138.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] NDUFA7 Rabbit pAb (STJ110739) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.")
Anti-NDUFA7 antibody (1-113) (STJ110739)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NDUFA7 (1-113) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NDUFA7 |
| Gene ID | 4701 |
| Uniprot ID | NDUA7_HUMAN |
| Immunogen Region | 1-113 |
| Immunogen Sequence | MASATRLIQRLRNWASGHDL QGKLQLRYQEISKRTQPPPK LPVGPSHKLSNNYYCTRDGR RESVPPSIIMSSQKALVSGK PAESSAVAATEKKAVTPAPP IKRWELSSDQPYL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-113 of human NDUFA7 (NP_004992.2). |
Additional Info
| Function | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. |
| Protein Name | Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Subunit 7Complex I-B14.5aCi-B14.5aNadh-Ubiquinone Oxidoreductase Subunit B14.5a |
| Database Links | Reactome: R-HSA-611105Reactome: R-HSA-6799198 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane ProteinMatrix Side |
| Alternative Antibody Names | Anti-Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Subunit 7 antibodyAnti-Complex I-B14.5a antibodyAnti-Ci-B14.5a antibodyAnti-Nadh-Ubiquinone Oxidoreductase Subunit B14.5a antibodyAnti-NDUFA7 antibody |
Information sourced from Uniprot.org