• Western blot analysis of extracts of various cell lines, using NDUFA6 antibody (STJ111873) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using NDUFA6 antibody (STJ111873) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NDUFA6 antibody (35-154) (STJ111873)

SKU:
STJ111873

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDUFA6 (35-154) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFA6
Gene ID: 4700
Uniprot ID: NDUA6_HUMAN
Immunogen Region: 35-154
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 35-154 of human NDUFA6 (NP_002481.2).
Immunogen Sequence: ATSTASTFVKPIFSRDMNEA KRRVRELYRAWYREVPNTVH QFQLDITVKMGRDKVREMFM KNAHVTDPRVVDLLVIKGKI ELEETIKVWKQRTHVMRFFH ETEAPRPKDFLSKFYVGHDP
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Required for proper complex I assembly. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protein Name Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Subunit 6
Complex I-B14
Ci-B14
Lyr Motif-Containing Protein 6
Nadh-Ubiquinone Oxidoreductase B14 Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Subunit 6 antibody
Anti-Complex I-B14 antibody
Anti-Ci-B14 antibody
Anti-Lyr Motif-Containing Protein 6 antibody
Anti-Nadh-Ubiquinone Oxidoreductase B14 Subunit antibody
Anti-NDUFA6 antibody
Anti-LYRM6 antibody
Anti-NADHB14 antibody

Information sourced from Uniprot.org

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