• Western blot analysis of extracts of various cell lines, using NDUFA12 antibody (STJ110536) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using NDUFA12 antibody (STJ110536) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NDUFA12 antibody (1-145) (STJ110536)

SKU:
STJ110536

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDUFA12 (1-145) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFA12
Gene ID: 55967
Uniprot ID: NDUAC_HUMAN
Immunogen Region: 1-145
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human NDUFA12 (NP_061326.1).
Immunogen Sequence: MELVQVLKRGLQQITGHGGL RGYLRVFFRTNDAKVGTLVG EDKYGNKYYEDNKQFFGRHR WVVYTTEMNGKNTFWDVDGS MVPPEWHRWLHSMTDDPPTT KPLTARKFIWTNHKFNVTGT PEQYVPYSTTRKKIQEWIPP STPYK
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protein Name Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Subunit 12
13 Kda Differentiation-Associated Protein
Complex I-B17.2
Ci-B17.2
Cib17.2
Nadh-Ubiquinone Oxidoreductase Subunit B17.2
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Subunit 12 antibody
Anti-13 Kda Differentiation-Associated Protein antibody
Anti-Complex I-B17.2 antibody
Anti-Ci-B17.2 antibody
Anti-Cib17.2 antibody
Anti-Nadh-Ubiquinone Oxidoreductase Subunit B17.2 antibody
Anti-NDUFA12 antibody
Anti-DAP13 antibody

Information sourced from Uniprot.org

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