Anti-NDST1 antibody (STJA0007711)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human NDST1
General Information
| Short Description | Rabbit polyclonal anti-Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NDST1 |
| Gene ID | 3340 |
| Uniprot ID | NDST1_HUMAN |
| Immunogen | Synthesized peptide derived from human NDST1 |
| Specificity | This antibody detects endogenous levels of NDST1 at Human, Mouse, Rat |
Additional Info
| Function | Isoform 1: Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response. Required for the exosomal release of SDCBP, CD63 and syndecan. Isoform 3: Lacks both N-deacetylase and N-sulfotransferase activities. Acts as a dominant negative on isoform 1, likely by changing the composition of enzyme complexes responsible for elongation and modification of heparan sulfates. |
| Protein Name | Bifunctional Heparan Sulfate N-Deacetylase/N-Sulfotransferase 1Glucosaminyl N-Deacetylase/N-Sulfotransferase 1Ndst-1N-Heparan Sulfate Sulfotransferase 1N-Hsst 1Heparan Sulfate-Glucosamine N-Sulfotransferase 1Hsnst 1 Includes - Heparan Sulfate N-Deacetylase 1 - Heparan Sulfate N-Sulfotransferase 1 |
| Database Links | Reactome: R-HSA-2022928 |
| Cellular Localisation | Isoform 1: Golgi ApparatusTrans-Golgi Network MembraneSingle-Pass Type Ii Membrane ProteinGolgi ApparatusCis-Golgi Network MembraneIsoform 3: Golgi Apparatus |
| Alternative Antibody Names | Anti-Bifunctional Heparan Sulfate N-Deacetylase/N-Sulfotransferase 1 antibodyAnti-Glucosaminyl N-Deacetylase/N-Sulfotransferase 1 antibodyAnti-Ndst-1 antibodyAnti-N-Heparan Sulfate Sulfotransferase 1 antibodyAnti-N-Hsst 1 antibodyAnti-Heparan Sulfate-Glucosamine N-Sulfotransferase 1 antibodyAnti-Hsnst 1 Includes - Heparan Sulfate N-Deacetylase 1 - Heparan Sulfate N-Sulfotransferase 1 antibodyAnti-NDST1 antibodyAnti-HSST antibodyAnti-HSST1 antibody |
Information sourced from Uniprot.org