• Western blot analysis of PC-3, using NDP antibody (STJ24710) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NDP antibody (25-133) (STJ24710)

SKU:
STJ24710

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDP (25-133) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDP
Gene ID: 4693
Uniprot ID: NDP_HUMAN
Immunogen Region: 25-133
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-133 of human NDP (NP_000257.1).
Immunogen Sequence: KTDSSFIMDSDPRRCMRHHY VDSISHPLYKCSSKMVLLAR CEGHCSQASRSEPLVSFSTV LKQPFRSSCHCCRPQTSKLK ALRLRCSGGMRLTATYRYIL SCHCEECNS
Tissue Specificity Expressed in the outer nuclear, inner nuclear and ganglion cell layers of the retina, and in fetal and adult brain.
Function Activates the canonical Wnt signaling pathway through FZD4 and LRP5 coreceptor. Plays a central role in retinal vascularization by acting as a ligand for FZD4 that signals via stabilizing beta-catenin (CTNNB1) and activating LEF/TCF-mediated transcriptional programs. Acts in concert with TSPAN12 to activate FZD4 independently of the Wnt-dependent activation of FZD4, suggesting the existence of a Wnt-independent signaling that also promote accumulation the beta-catenin (CTNNB1). May be involved in a pathway that regulates neural cell differentiation and proliferation. Possible role in neuroectodermal cell-cell interaction.
Protein Name Norrin
Norrie Disease Protein
X-Linked Exudative Vitreoretinopathy 2 Protein
Cellular Localisation Secreted
Alternative Antibody Names Anti-Norrin antibody
Anti-Norrie Disease Protein antibody
Anti-X-Linked Exudative Vitreoretinopathy 2 Protein antibody
Anti-NDP antibody
Anti-EVR2 antibody

Information sourced from Uniprot.org

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