• Western blot analysis of extracts of various cell lines, using HEC1/NDC80 Rabbit monoclonal antibody (STJ11101816) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-NDC80 antibody (543-642) [S6MR] (STJ11101816)

SKU:
STJ11101816

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-HEC1 (543-642) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDC80
Gene ID: 10403
Uniprot ID: NDC80_HUMAN
Immunogen Region: 543-642
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 543-642 of human HEC1/NDC80 (O14777).
Immunogen Sequence: ESTVNQGLSEAMNELDAVQR EYQLVVQTTTEERRKVGNNL QRLLEMVATHVGSVEKHLEE QIAKVDREYEECMSEDLSEN IKEIRDKYEKKATLIKSSEE
Post Translational Modifications Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. Also phosphorylated by AURKA and AURKB. Acetylated at Lys-53 and Lys-59 by KAT5 during mitosis, promoting robust kinetochore-microtubule attachment. Deacetylated by SIRT1.
Function Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules.
Protein Name Kinetochore Protein Ndc80 Homolog
Highly Expressed In Cancer Protein
Kinetochore Protein Hec1
Hshec1
Kinetochore-Associated Protein 2
Retinoblastoma-Associated Protein Hec
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5663220
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Cellular Localisation Nucleus
Chromosome
Centromere
Kinetochore
Localizes To Kinetochores From Late Prophase To Anaphase
Localizes Specifically To The Outer Plate Of The Kinetochore
Alternative Antibody Names Anti-Kinetochore Protein Ndc80 Homolog antibody
Anti-Highly Expressed In Cancer Protein antibody
Anti-Kinetochore Protein Hec1 antibody
Anti-Hshec1 antibody
Anti-Kinetochore-Associated Protein 2 antibody
Anti-Retinoblastoma-Associated Protein Hec antibody
Anti-NDC80 antibody
Anti-HEC antibody
Anti-HEC1 antibody
Anti-KNTC2 antibody

Information sourced from Uniprot.org

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