• Immunofluorescence analysis of U2OS cells using NCSTN Rabbit polyclonal antibody (STJ116716) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using NCSTN Rabbit polyclonal antibody (STJ116716) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using NCSTN Rabbit polyclonal antibody (STJ116716) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NCSTN antibody (429-669) (STJ116716)

SKU:
STJ116716

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NCSTN (429-669) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NCSTN
Gene ID: 23385
Uniprot ID: NICA_HUMAN
Immunogen Region: 429-669
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 429-669 of human NCSTN (NP_056146.1).
Immunogen Sequence: RFLRARNISGVVLADHSGAF HNKYYQSIYDTAENINVSYP EWLSPEEDLNFVTDTAKALA DVATVLGRALYELAGGTNFS DTVQADPQTVTRLLYGFLIK ANNSWFQSILRQDLRSYLGD GPLQHYIAVSSPTNTTYVVQ YALANLTGTVVNLTREQCQD PSKVPSENKDLYEYSWVQGP LHSNETDRLPRCVRSTARLA RALSPAFELSQWSSTEYSTW TESRWKDIRARIFLIASKE
Tissue Specificity Detected in brain (at protein level). Widely expressed.
Post Translational Modifications N-glycosylated.
Function Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels.
Protein Name Nicastrin
Database Links Reactome: R-HSA-1251985
Reactome: R-HSA-1474228
Reactome: R-HSA-193692
Reactome: R-HSA-205043
Reactome: R-HSA-2122948
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-2979096
Reactome: R-HSA-3928665
Reactome: R-HSA-6798695
Reactome: R-HSA-9013507
Reactome: R-HSA-9013700
Reactome: R-HSA-9017802
Reactome: R-HSA-977225
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Cytoplasmic Vesicle Membrane
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Nicastrin antibody
Anti-NCSTN antibody
Anti-KIAA0253 antibody
Anti-UNQ1874 antibody
Anti-PRO4317 antibody

Information sourced from Uniprot.org

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