• Western blot analysis of lysates from HepG2 and HUVEC cells, using NCLN Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-NCLN antibody (474-523) (STJ94489)

SKU:
STJ94489

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BOS complex subunit NCLN (474-523) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NCLN
Gene ID: 56926
Uniprot ID: NCLN_HUMAN
Immunogen Region: 474-523
Specificity: Nicalin Polyclonal Antibody detects endogenous levels of Nicalin protein.
Immunogen: The antiserum was produced against synthesized peptide derived from human NCLN. AA range:474-523
Tissue Specificity Highly expressed in pancreas and skeletal muscle and, at lower levels, in heart.
Function Component of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer of membranes. The MPT complex takes over after the SEC61 complex: following membrane insertion of the first few transmembrane segments of proteins by the SEC61 complex, the MPT complex occludes the lateral gate of the SEC61 complex to promote insertion of subsequent transmembrane regions. May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning, via its interaction with NOMO.
Protein Name Bos Complex Subunit Ncln
Nicalin
Nicastrin-Like Protein
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Bos Complex Subunit Ncln antibody
Anti-Nicalin antibody
Anti-Nicastrin-Like Protein antibody
Anti-NCLN antibody

Information sourced from Uniprot.org

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