Anti-NCLN antibody (474-523) (STJ94489)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human NCLN. AA range:474-523
General Information
| Short Description | Rabbit polyclonal anti-BOS complex subunit NCLN (474-523) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NCLN |
| Gene ID | 56926 |
| Uniprot ID | NCLN_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NCLN. AA range:474-523 |
| Immunogen Region | 474-523 |
| Specificity | Nicalin Polyclonal Antibody detects endogenous levels of Nicalin protein. |
Additional Info
| Tissue Specificity | Highly expressed in pancreas and skeletal muscle and, at lower levels, in heart. |
| Function | Component of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer of membranes. The MPT complex takes over after the SEC61 complex: following membrane insertion of the first few transmembrane segments of proteins by the SEC61 complex, the MPT complex occludes the lateral gate of the SEC61 complex to promote insertion of subsequent transmembrane regions. May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning, via its interaction with NOMO. |
| Protein Name | Bos Complex Subunit NclnNicalinNicastrin-Like Protein |
| Database Links | |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Bos Complex Subunit Ncln antibodyAnti-Nicalin antibodyAnti-Nicastrin-Like Protein antibodyAnti-NCLN antibody |
Information sourced from Uniprot.org