Anti-NCKAP1 antibody (1-250) (STJ114120)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NCKAP1 (1-250) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NCKAP1 |
| Gene ID | 10787 |
| Uniprot ID | NCKP1_HUMAN |
| Immunogen Region | 1-250 |
| Immunogen Sequence | MSRSVLQPSQQKLAEKLTIL NDRGVGMLTRLYNIKKACGD PKAKPSYLIDKNLESAVKFI VRKFPAVETRNNNQQLAQLQ KEKSEILKNLALYYFTFVDV MEFKDHVCELLNTIDVCQVF FDITVNFDLTKNYLDLIITY TTLMILLSRIEERKAIIGLY NYAHEMTHGASDREYPRLGQ MIVDYENPLKKMMEEFVPHS KSLSDALISLQMVYPRRNLS ADQWRNAQLLSLISAPSTM |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NCKAP1 (NP_038464.1). |
Additional Info
| Tissue Specificity | Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle. Expressed in cells of various brain regions including Purkinje cells and dentate nucleus of the cerebellum, CA4 region and dentate gyrus of the hippocampus, and in frontal gray and white matter. |
| Function | Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes. |
| Protein Name | Nck-Associated Protein 1Nap 1Membrane-Associated Protein Hem-2P125nap1 |
| Database Links | Reactome: R-HSA-2029482Reactome: R-HSA-4420097Reactome: R-HSA-5663213Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013423Reactome: R-HSA-9664422 |
| Cellular Localisation | Cell MembraneSingle-Pass Membrane ProteinCytoplasmic SideCell ProjectionLamellipodium MembraneAt The Interface Between The Lamellipodial Actin Meshwork And The Membrane |
| Alternative Antibody Names | Anti-Nck-Associated Protein 1 antibodyAnti-Nap 1 antibodyAnti-Membrane-Associated Protein Hem-2 antibodyAnti-P125nap1 antibodyAnti-NCKAP1 antibodyAnti-HEM2 antibodyAnti-KIAA0587 antibodyAnti-NAP1 antibody |
Information sourced from Uniprot.org