• Immunohistochemistry analysis of paraffin-embedded human prostate using NCK2 antibody (STJ110061) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using NCK2 antibody (STJ110061) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-NCK2 antibody (1-380) (STJ110061)

SKU:
STJ110061

£169.50 - £449.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NCK2 (1-380) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NCK2
Gene ID: 8440
Uniprot ID: NCK2_HUMAN
Immunogen Region: 1-380
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-380 of human NCK2 (NP_003572.2).
Immunogen Sequence: MTEEVIVIAKWDYTAQQDQE LDIKKNERLWLLDDSKTWWR VRNAANRTGYVPSNYVERKN SLKKGSLVKNLKDTLGLGKT RRKTSARDASPTPSTDAEYP ANGSGADRIYDLNIPAFVKF AYVAEREDELSLVKGSRVTV MEKCSDGWWRGSYNGQIGWF PSNYVLEEVDEAAAESPSFL SLRKGASLSNGQGSRVLHVV QTLYPFSSVTEEELNFEKGE TMEVIEKPENDPEWWKCKN
Tissue Specificity Ubiquitous.
Post Translational Modifications Phosphorylated.
Function Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.
Protein Name Cytoplasmic Protein Nck2
Growth Factor Receptor-Bound Protein 4
Nck Adaptor Protein 2
Nck-2
Sh2/Sh3 Adaptor Protein Nck-Beta
Database Links Reactome: R-HSA-186763
Reactome: R-HSA-373753
Reactome: R-HSA-3928664
Reactome: R-HSA-428540
Reactome: R-HSA-4420097
Reactome: R-HSA-8985801
Reactome: R-HSA-9013420
Reactome: R-HSA-9013424
Cellular Localisation Cytoplasm
Endoplasmic Reticulum
Alternative Antibody Names Anti-Cytoplasmic Protein Nck2 antibody
Anti-Growth Factor Receptor-Bound Protein 4 antibody
Anti-Nck Adaptor Protein 2 antibody
Anti-Nck-2 antibody
Anti-Sh2/Sh3 Adaptor Protein Nck-Beta antibody
Anti-NCK2 antibody
Anti-GRB4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance