| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-Nck (200-300) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S0MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NCK1 |
| Gene ID: |
4690 |
| Uniprot ID: |
NCK1_HUMAN |
| Immunogen Region: |
200-300 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human NCK11 (P16333). |
| Immunogen Sequence: |
PFSSSNDEELNFEKGDVMDV IEKPENDPEWWKCRKINGMV GLVPKNYVTVMQNNPLTSGL EPSPPQCDYIRPSLTGKFAG NPWYYGKVTRHQAEMALNER G |
| Post Translational Modifications | Phosphorylated on Ser and Tyr residues. Phosphorylated in response to activation of EGFR and FcERI. Phosphorylated by activated PDGFRB. |
| Function | Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors. |
| Protein Name | Cytoplasmic Protein Nck1Nck Adaptor Protein 1Nck-1Sh2/Sh3 Adaptor Protein Nck-Alpha |
| Database Links | Reactome: R-HSA-186763Reactome: R-HSA-202433Reactome: R-HSA-2029482Reactome: R-HSA-373753Reactome: R-HSA-418885Reactome: R-HSA-428540Reactome: R-HSA-4420097Reactome: R-HSA-5663213Reactome: R-HSA-9013420Reactome: R-HSA-9013424Reactome: R-HSA-9664422Reactome: R-HSA-9679191Reactome: R-HSA-983695 |
| Cellular Localisation | CytoplasmEndoplasmic ReticulumNucleusMostly CytoplasmicBut Shuttles Between The Cytoplasm And The NucleusImport Into The Nucleus Requires The Interaction With Socs7Predominantly Nuclear Following Genotoxic StressesSuch As Uv IrradiationHydroxyurea Or Mitomycin C Treatments |
| Alternative Antibody Names | Anti-Cytoplasmic Protein Nck1 antibodyAnti-Nck Adaptor Protein 1 antibodyAnti-Nck-1 antibodyAnti-Sh2/Sh3 Adaptor Protein Nck-Alpha antibodyAnti-NCK1 antibodyAnti-NCK antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
