• STJ70023 (0. 2µg/ml) staining of U937 (A) , (0. 01ug/ml) Daudi (B) , (0. 3ug/ml) U251 (C) and negative control A431 (D) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70023 (1µg/ml) staining of Mouse Thymus (A) and Pig Spleen (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70023 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70023 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-NCF1/p47phox antibody (C-Term) (STJ70023)

SKU:
STJ70023-100

€379.38
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Host: Goat
Applications: Pep-ELISA/WB/IHC/IF/FC
Reactivity: Human/Mouse/Rat/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-NCF1/p47phox (C-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:20000.
WB: Approx 48kDa band observed in lysates of cell lines Daudi, U251 and U937 and in Mouse Thymus, and approx, 50kDa in Pig Spleen lysates (calculated MW of 44.7kDa according to Human NP_00
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: NCF1
Gene ID: 653361
Uniprot ID: NCF1_HUMAN
Immunogen Region: C-Term
Accession Number: NP_000256.4
Immunogen Sequence: SESTKRKLASAV
Post Translational Modifications Phosphorylated by PRKCD.phosphorylation induces activation of NCF1 and NADPH oxidase activity.
Function NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Protein Name Neutrophil Cytosol Factor 1
Ncf-1
47 Kda Autosomal Chronic Granulomatous Disease Protein
47 Kda Neutrophil Oxidase Factor
Ncf-47k
Neutrophil Nadph Oxidase Factor 1
Nox Organizer 2
Nox-Organizing Protein 2
Sh3 And Px Domain-Containing Protein 1a
P47-Phox
Database Links Reactome: R-HSA-1222556
Reactome: R-HSA-1236973
Reactome: R-HSA-3299685
Reactome: R-HSA-4420097
Reactome: R-HSA-5668599
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013423
Cellular Localisation Cytoplasm
Cytosol
Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Neutrophil Cytosol Factor 1 antibody
Anti-Ncf-1 antibody
Anti-47 Kda Autosomal Chronic Granulomatous Disease Protein antibody
Anti-47 Kda Neutrophil Oxidase Factor antibody
Anti-Ncf-47k antibody
Anti-Neutrophil Nadph Oxidase Factor 1 antibody
Anti-Nox Organizer 2 antibody
Anti-Nox-Organizing Protein 2 antibody
Anti-Sh3 And Px Domain-Containing Protein 1a antibody
Anti-P47-Phox antibody
Anti-NCF1 antibody
Anti-NOXO2 antibody
Anti-SH3PXD1A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance