Host: | Goat |
Applications: | Pep-ELISA/WB/IHC/IF/FC |
Reactivity: | Human/Mouse/Rat/Pig/Cow |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Goat polyclonal antibody anti-NCF1/p47phox (C-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Concentration: | 0.5 mg/mL |
Dilution Range: | IHC-5µg/ml IF-Strong expression of the protein seen in the cytoplasm of HeLa cells. 10µg/ml FC-Flow cytometric analysis of HeLa cells. 10ug/ml ELISA-antibody detection limit dilution 1:20000. |
Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Gene Symbol: | NCF1 |
Gene ID: | 653361 |
Uniprot ID: | NCF1_HUMAN |
Immunogen Region: | C-Term |
Accession Number: | NP_000256.4 |
Immunogen Sequence: | SESTKRKLASAV |
Post Translational Modifications | Phosphorylated by PRKCD.phosphorylation induces activation of NCF1 and NADPH oxidase activity. |
Function | NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). |
Protein Name | Neutrophil Cytosol Factor 1 Ncf-1 47 Kda Autosomal Chronic Granulomatous Disease Protein 47 Kda Neutrophil Oxidase Factor Ncf-47k Neutrophil Nadph Oxidase Factor 1 Nox Organizer 2 Nox-Organizing Protein 2 Sh3 And Px Domain-Containing Protein 1a P47-Phox |
Database Links | Reactome: R-HSA-1222556 Reactome: R-HSA-1236973 Reactome: R-HSA-3299685 Reactome: R-HSA-4420097 Reactome: R-HSA-5668599 Reactome: R-HSA-9013149 Reactome: R-HSA-9013404 Reactome: R-HSA-9013423 |
Cellular Localisation | Cytoplasm Cytosol Membrane Peripheral Membrane Protein Cytoplasmic Side |
Alternative Antibody Names | Anti-Neutrophil Cytosol Factor 1 antibody Anti-Ncf-1 antibody Anti-47 Kda Autosomal Chronic Granulomatous Disease Protein antibody Anti-47 Kda Neutrophil Oxidase Factor antibody Anti-Ncf-47k antibody Anti-Neutrophil Nadph Oxidase Factor 1 antibody Anti-Nox Organizer 2 antibody Anti-Nox-Organizing Protein 2 antibody Anti-Sh3 And Px Domain-Containing Protein 1a antibody Anti-P47-Phox antibody Anti-NCF1 antibody Anti-NOXO2 antibody Anti-SH3PXD1A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance