Anti-NCF1 antibody (1-390) (STJ24698)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NCF1 (1-390) antibody for use in WB, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:100IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NCF1 |
| Gene ID | 653361 |
| Uniprot ID | NCF1_HUMAN |
| Immunogen Region | 1-390 |
| Immunogen Sequence | MGDTFIRHIALLGFEKRFVP SQHYVYMFLVKWQDLSEKVV YRRFTEIYEFHKTLKEMFPI EAGAINPENRIIPHLPAPKW FDGQRAAENRQGTLTEYCST LMSLPTKISRCPHLLDFFKV RPDDLKLPTDNQTKKPETYL MPKDGKSTATDITGPIILQT YRAIANYEKTSGSEMALSTG DVVEVVEKSESGWWFCQMKA KRGWIPASFLEPLDSPDETE DPEPNYAGEPYVAIKAYTA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-390 of human p47phox/NCF1 (NP_000256.4). |
Additional Info
| Tissue Specificity | Detected in peripheral blood monocytes and neutrophils (at protein level). |
| Post Translational Modifications | Phosphorylated by PRKCD.phosphorylation induces activation of NCF1, leading to assembly and activation of the NADPH oxidase complex. |
| Function | Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction. Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane. This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox. |
| Protein Name | Neutrophil Cytosol Factor 1Ncf-147 Kda Autosomal Chronic Granulomatous Disease Protein47 Kda Neutrophil Oxidase FactorNcf-47kNeutrophil Nadph Oxidase Factor 1Nox Organizer 2Nox-Organizing Protein 2Sh3 And Px Domain-Containing Protein 1aP47-Phox |
| Database Links | Reactome: R-HSA-1222556Reactome: R-HSA-1236973Reactome: R-HSA-3299685Reactome: R-HSA-4420097Reactome: R-HSA-5668599Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013423 |
| Cellular Localisation | CytoplasmCytosolMembranePeripheral Membrane ProteinCytoplasmic Side |
| Alternative Antibody Names | Anti-Neutrophil Cytosol Factor 1 antibodyAnti-Ncf-1 antibodyAnti-47 Kda Autosomal Chronic Granulomatous Disease Protein antibodyAnti-47 Kda Neutrophil Oxidase Factor antibodyAnti-Ncf-47k antibodyAnti-Neutrophil Nadph Oxidase Factor 1 antibodyAnti-Nox Organizer 2 antibodyAnti-Nox-Organizing Protein 2 antibodyAnti-Sh3 And Px Domain-Containing Protein 1a antibodyAnti-P47-Phox antibodyAnti-NCF1 antibodyAnti-NOXO2 antibodyAnti-SH3PXD1A antibody |
Information sourced from Uniprot.org