• Anti-NCBP1 antibody (1-100) (STJ115874)
  • Immunohistochemistry analysis of paraffin-embedded human brain using CBP80 Rabbit polyclonal antibody (STJ115874) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CBP80 antibody (STJ115874) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-NCBP1 antibody (1-100) (STJ115874)

SKU:
STJ115874

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CBP80 (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NCBP1
Gene ID: 4686
Uniprot ID: NCBP1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CBP80 (NP_002477.1).
Immunogen Sequence: MSRRRHSDENDGGQPHKRRK TSDANETEDHLESLICKVGE KSACSLESNLEGLAGVLEAD LPNYKSKILRLLCTVARLLP EKLTIYTTLVGLLNARNYNF
Function Component of the cap-binding complex (CBC), which binds cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5'-end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure. Associates with NCBP3 to form an alternative cap-binding complex (CBC) which plays a key role in mRNA export and is particularly important in cellular stress situations such as virus infections. The conventional CBC with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus whereas the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role only in mRNA export. NCBP1/CBP80 is required for cell growth and viability.
Protein Name Nuclear Cap-Binding Protein Subunit 1
80 Kda Nuclear Cap-Binding Protein
Cbp80
Ncbp 80 Kda Subunit
Database Links Reactome: R-HSA-111367
Reactome: R-HSA-112382
Reactome: R-HSA-113418
Reactome: R-HSA-159227
Reactome: R-HSA-159230
Reactome: R-HSA-159231
Reactome: R-HSA-159236
Reactome: R-HSA-167152
Reactome: R-HSA-167158
Reactome: R-HSA-167200
Reactome: R-HSA-167242
Reactome: R-HSA-191859
Reactome: R-HSA-674695
Reactome: R-HSA-6803529
Reactome: R-HSA-6807505
Reactome: R-HSA-72086
Reactome: R-HSA-72163
Reactome: R-HSA-72165
Reactome: R-HSA-72187
Reactome: R-HSA-72203
Reactome: R-HSA-73856
Reactome: R-HSA-77588
Reactome: R-HSA-77595
Reactome: R-HSA-8851708
Reactome: R-HSA-9010553
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Nucleus
Cytoplasm
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Alternative Antibody Names Anti-Nuclear Cap-Binding Protein Subunit 1 antibody
Anti-80 Kda Nuclear Cap-Binding Protein antibody
Anti-Cbp80 antibody
Anti-Ncbp 80 Kda Subunit antibody
Anti-NCBP1 antibody
Anti-CBP80 antibody
Anti-NCBP antibody

Information sourced from Uniprot.org

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