Anti-NCAPH antibody (441-490 aa) (STJ93471)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human NCAPH at the amino acid range 441-490
STJ93471
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General Information

Short DescriptionRabbit polyclonal anti-Condensin complex subunit 2 (441-490 aa) for use in WB and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Rat/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:500-1:2000
ELISA 1:40000
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolNCAPH
Gene ID23397
Uniprot IDCND2_HUMAN
ImmunogenThe antiserum was produced against synthesized peptide derived from the human NCAPH at the amino acid range 441-490
Immunogen Region441-490 aa
SpecificityhCAP-H Polyclonal Antibody detects endogenous levels of hCAP-H protein.

Additional Info

Post Translational Modifications Phosphorylated by CDK1. Its phosphorylation, as well as that of NCAPD2 and NCAPG subunits, activates the condensin complex and is required for chromosome condensation.
Function Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size.
Protein Name Condensin Complex Subunit 2
Barren Homolog Protein 1
Chromosome-Associated Protein H
Hcap-H
Non-Smc Condensin I Complex Subunit H
Xcap-H Homolog
Database Links Reactome: R-HSA-2514853
Cellular Localisation Nucleus
Cytoplasm
Chromosome
In Interphase Cells
The Majority Of The Condensin Complex Is Found In The Cytoplasm
While A Minority Of The Complex Is Associated With Chromatin
A Subpopulation Of The Complex However Remains Associated With Chromosome Foci In Interphase Cells
During Mitosis
Most Of The Condensin Complex Is Associated With The Chromatin
At The Onset Of Prophase
The Regulatory Subunits Of The Complex Are Phosphorylated By Cdk1
Leading To Condensin's Association With Chromosome Arms And To Chromosome Condensation
Dissociation From Chromosomes Is Observed In Late Telophase
Alternative Antibody Names Anti-Condensin Complex Subunit 2 antibody
Anti-Barren Homolog Protein 1 antibody
Anti-Chromosome-Associated Protein H antibody
Anti-Hcap-H antibody
Anti-Non-Smc Condensin I Complex Subunit H antibody
Anti-Xcap-H Homolog antibody
Anti-NCAPH antibody
Anti-BRRN antibody
Anti-BRRN1 antibody
Anti-CAPH antibody
Anti-KIAA0074 antibody

Information sourced from Uniprot.org

Citations

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