• Western blot analysis of lysates from COLO cells, using NCAPH Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of the lysates from COLO205 cells using NCAPH antibody.
  • Western blot analysis of COLO cells using hCAP-H Polyclonal Antibody

Anti-NCAPH antibody (441-490 aa) (STJ93471)

SKU:
STJ93471

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Condensin complex subunit 2 (441-490 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NCAPH
Gene ID: 23397
Uniprot ID: CND2_HUMAN
Immunogen Region: 441-490 aa
Specificity: hCAP-H Polyclonal Antibody detects endogenous levels of hCAP-H protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human NCAPH at the amino acid range 441-490
Post Translational Modifications Phosphorylated by CDK1. Its phosphorylation, as well as that of NCAPD2 and NCAPG subunits, activates the condensin complex and is required for chromosome condensation.
Function Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size.
Protein Name Condensin Complex Subunit 2
Barren Homolog Protein 1
Chromosome-Associated Protein H
Hcap-H
Non-Smc Condensin I Complex Subunit H
Xcap-H Homolog
Database Links Reactome: R-HSA-2514853
Cellular Localisation Nucleus
Cytoplasm
Chromosome
In Interphase Cells
The Majority Of The Condensin Complex Is Found In The Cytoplasm
While A Minority Of The Complex Is Associated With Chromatin
A Subpopulation Of The Complex However Remains Associated With Chromosome Foci In Interphase Cells
During Mitosis
Most Of The Condensin Complex Is Associated With The Chromatin
At The Onset Of Prophase
The Regulatory Subunits Of The Complex Are Phosphorylated By Cdk1
Leading To Condensin's Association With Chromosome Arms And To Chromosome Condensation
Dissociation From Chromosomes Is Observed In Late Telophase
Alternative Antibody Names Anti-Condensin Complex Subunit 2 antibody
Anti-Barren Homolog Protein 1 antibody
Anti-Chromosome-Associated Protein H antibody
Anti-Hcap-H antibody
Anti-Non-Smc Condensin I Complex Subunit H antibody
Anti-Xcap-H Homolog antibody
Anti-NCAPH antibody
Anti-BRRN antibody
Anti-BRRN1 antibody
Anti-CAPH antibody
Anti-KIAA0074 antibody

Information sourced from Uniprot.org

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