Anti-NCAPH antibody (1-250) (STJ24697)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NCAPH (1-250) antibody for use in WB, IF, ICC and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:5000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NCAPH |
| Gene ID | 23397 |
| Uniprot ID | CND2_HUMAN |
| Immunogen Region | 1-250 |
| Immunogen Sequence | MGPPGPALPATMNNSSSETR GHPHSASSPSERVFPMPLPR KAPLNIPGTPVLEDFPQNDD EKERLQRRRSRVFDLQFSTD SPRLLASPSSRSIDISATIP KFTNTQITEHYSTCIKLSTE NKITTKNAFGLHLIDFMSEI LKQKDTEPTNFKVAAGTLDA STKIYAVRVDAVHADVYRVL GGLGKDAPSLEEVEGHVADG SATEMGTTKKAVKPKKKHLH RTIEQNINNLNVSEADRKC |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NCAPH (NP_056156.2). |
Additional Info
| Tissue Specificity | Widely expressed at low level. Expressed in proliferating cells. |
| Post Translational Modifications | Phosphorylated by CDK1. Its phosphorylation, as well as that of NCAPD2 and NCAPG subunits, activates the condensin complex and is required for chromosome condensation. |
| Function | Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size. |
| Protein Name | Condensin Complex Subunit 2Barren Homolog Protein 1Chromosome-Associated Protein HHcap-HNon-Smc Condensin I Complex Subunit HXcap-H Homolog |
| Database Links | Reactome: R-HSA-2514853 |
| Cellular Localisation | NucleusCytoplasmChromosomeIn Interphase CellsThe Majority Of The Condensin Complex Is Found In The CytoplasmWhile A Minority Of The Complex Is Associated With ChromatinA Subpopulation Of The Complex However Remains Associated With Chromosome Foci In Interphase CellsDuring MitosisMost Of The Condensin Complex Is Associated With The ChromatinAt The Onset Of ProphaseThe Regulatory Subunits Of The Complex Are Phosphorylated By Cdk1Leading To Condensin's Association With Chromosome Arms And To Chromosome CondensationDissociation From Chromosomes Is Observed In Late Telophase |
| Alternative Antibody Names | Anti-Condensin Complex Subunit 2 antibodyAnti-Barren Homolog Protein 1 antibodyAnti-Chromosome-Associated Protein H antibodyAnti-Hcap-H antibodyAnti-Non-Smc Condensin I Complex Subunit H antibodyAnti-Xcap-H Homolog antibodyAnti-NCAPH antibodyAnti-BRRN antibodyAnti-BRRN1 antibodyAnti-CAPH antibodyAnti-KIAA0074 antibody |
Information sourced from Uniprot.org