• Western blot analysis of extracts of various cell lines, using NCALD antibody (STJ110307) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using NCALD antibody (STJ110307) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NCALD antibody (1-193) (STJ110307)

SKU:
STJ110307

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NCALD (1-193) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NCALD
Gene ID: 83988
Uniprot ID: NCALD_HUMAN
Immunogen Region: 1-193
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human NCALD (NP_114430.2).
Immunogen Sequence: MGKQNSKLRPEVMQDLLEST DFTEHEIQEWYKGFLRDCPS GHLSMEEFKKIYGNFFPYGD ASKFAEHVFRTFDANGDGTI DFREFIIALSVTSRGKLEQK LKWAFSMYDLDGNGYISKAE MLEIVQAIYKMVSSVMKMPE DESTPEKRTEKIFRQMDTNR DGKLSLEEFIRGAKSDPSIV RLLQCDPSSAGQF
Tissue Specificity Retina, cerebrum, cerebellum, brain stem, spinal cord, testis, ovary and small intestine.
Function May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds three calcium ions.
Protein Name Neurocalcin-Delta
Database Links Reactome: R-HSA-451308
Alternative Antibody Names Anti-Neurocalcin-Delta antibody
Anti-NCALD antibody

Information sourced from Uniprot.org

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