• Western blot analysis of lysates from wild type (WT) and NANS knockout (KO) 293T cells, using [KO Validated] NANS Rabbit polyclonal antibody (STJ11100751) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-NANS antibody (260-359) (STJ11100751)

SKU:
STJ11100751

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NANS (260-359) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NANS
Gene ID: 54187
Uniprot ID: SIAS_HUMAN
Immunogen Region: 260-359
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 260-359 of human NANS (NP_061819.2).
Immunogen Sequence: AELVRSVRLVERALGSPTKQ LLPCEMACNEKLGKSVVAKV KIPEGTILTMDMLTVKVGEP KGYPPEDIFNLVGKKVLVTV EEDDTIMEELVDNHGKKIKS
Tissue Specificity Ubiquitous.
Function Produces N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN). Can also use N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of Neu5Ac and KDN, respectively.
Protein Name Sialic Acid Synthase
N-Acetylneuraminate Synthase
N-Acetylneuraminate-9-Phosphate Synthase
N-Acetylneuraminic Acid Phosphate Synthase
N-Acetylneuraminic Acid Synthase
Database Links Reactome: R-HSA-4085001
Alternative Antibody Names Anti-Sialic Acid Synthase antibody
Anti-N-Acetylneuraminate Synthase antibody
Anti-N-Acetylneuraminate-9-Phosphate Synthase antibody
Anti-N-Acetylneuraminic Acid Phosphate Synthase antibody
Anti-N-Acetylneuraminic Acid Synthase antibody
Anti-NANS antibody
Anti-SAS antibody

Information sourced from Uniprot.org

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