Anti-Nanog antibody [SRM14858] (STJ11109588)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein (or fragment).This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit monoclonal Nanog antibody for use in ELISA, WB, ChIP and FC in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB/ChIP/FC |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM14858 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB, 1:500-1:1000ChIP, 5 Mu g antibody for 10 Mu g-15 Mu g of ChromatinFC (intra) , 1:500-1:1000 |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Nanog |
| Gene ID | 71950 |
| Uniprot ID | NANOG_MOUSE |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
Additional Info
| Tissue Specificity | Not expressed in oocytes and spermatogonia (at protein level). Not expressed in many somatic organs, ovary, testis, fibroblast and hematopoietic cell lines. |
| Function | Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAATGTGT-3' or 5'-CGGACGCGCATTANGC-3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation. |
| Protein Name | Homeobox Protein NanogEs Cell-Associated Protein 4Early Embryo Specific Expression Nk-Type Homeobox ProteinHomeobox Transcription Factor Nanog |
| Database Links | |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Homeobox Protein Nanog antibodyAnti-Es Cell-Associated Protein 4 antibodyAnti-Early Embryo Specific Expression Nk-Type Homeobox Protein antibodyAnti-Homeobox Transcription Factor Nanog antibodyAnti-Nanog antibodyAnti-Ecat4 antibodyAnti-Enk antibody |
Information sourced from Uniprot.org