Anti-Nanog antibody [PT0179R] (STJA0039699)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgGk
General Information
| Short Description | Rabbit monoclonal anti-Homeobox Protein Nanog for use in WB, IHC, IF and ELISA in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | PT0179R |
| Isotype | IgGk |
| Conjugation | Unconjugated |
| Purification | Protein A |
| Dilution Range | IHC 1:200-1000WB 1:1000-5000IF 1:200-1000ELISA 1:5000-20000 |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NANOG |
| Gene ID | 79923 |
| Uniprot ID | NANOG_HUMAN |
| Specificity | Endogenous |
Additional Info
| Function | Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAATGTGT-3' or 5'-CGGACGCGCATTANGC-3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation. |
| Protein Name | Homeobox Protein NanogHomeobox Transcription Factor NanogHnanog |
| Database Links | Reactome: R-HSA-2892245Reactome: R-HSA-2892247Reactome: R-HSA-452723Reactome: R-HSA-6785807Reactome: R-HSA-9754189Reactome: R-HSA-9823739Reactome: R-HSA-9827857 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Homeobox Protein Nanog antibodyAnti-Homeobox Transcription Factor Nanog antibodyAnti-Hnanog antibodyAnti-NANOG antibody |
Information sourced from Uniprot.org