| Host: |
Mouse |
| Applications: |
WB/IF/ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Homeobox protein NANOG (20-166 aa) is suitable for use in Western Blot, Immunofluorescence and ELISA research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
1E6C4 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF 1:200-1:1000ELISA 1:10000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NANOG |
| Gene ID: |
79923 |
| Uniprot ID: |
NANOG_HUMAN |
| Immunogen Region: |
20-166 aa |
| Specificity: |
Nanog Monoclonal Antibody detects endogenous levels of Nanog protein. |
| Immunogen: |
Purified recombinant fragment of Nanog (aa20-166) expressed in E. Coli. |
| Function | Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAATGTGT-3' or 5'-CGGACGCGCATTANGC-3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation. |
| Protein Name | Homeobox Protein NanogHomeobox Transcription Factor NanogHnanog |
| Database Links | Reactome: R-HSA-2892245Reactome: R-HSA-2892247Reactome: R-HSA-452723Reactome: R-HSA-6785807Reactome: R-HSA-9754189Reactome: R-HSA-9827857 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Homeobox Protein Nanog antibodyAnti-Homeobox Transcription Factor Nanog antibodyAnti-Hnanog antibodyAnti-NANOG antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
