• Western blot analysis of extracts of various cell lines, using NAB2 antibody (STJ28390) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NAB2 antibody (1-200) (STJ28390)

SKU:
STJ28390

$151.88 - $705.38
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NAB2 (1-200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NAB2
Gene ID: 4665
Uniprot ID: NAB2_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human NAB2 (NP_005958.1).
Immunogen Sequence: MHRAPSPTAEQPPGGGDSAR RTLQPRLKPSARAMALPRTL GELQLYRVLQRANLLSYYET FIQQGGDDVQQLCEAGEEEF LEIMALVGMATKPLHVRRLQ KALREWATNPGLFSQPVPAV PVSSIPLFKISETAGTRKGS MSNGHGSPGEKAGSARSFSP KSPLELGEKLSPLPGGPGAG DPRIWPGRSTPESDVGAGGE
Tissue Specificity Widely expressed at low levels. Highly expressed in melanoma cell lines.
Post Translational Modifications Sumoylation by EGR2 represses EGR2 transcriptional activity in hindbrain.
Function Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability.
Protein Name Ngfi-A-Binding Protein 2
Egr-1-Binding Protein 2
Melanoma-Associated Delayed Early Response Protein
Protein Mader
Database Links Reactome: R-HSA-9031628
Reactome: R-HSA-9619665
Cellular Localisation Nucleus
Isoform 2 Is Not Localized To The Nucleus
Alternative Antibody Names Anti-Ngfi-A-Binding Protein 2 antibody
Anti-Egr-1-Binding Protein 2 antibody
Anti-Melanoma-Associated Delayed Early Response Protein antibody
Anti-Protein Mader antibody
Anti-NAB2 antibody
Anti-MADER antibody

Information sourced from Uniprot.org

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