Anti-ELP3 antibody (1-200) (STJ111919)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ELP3 (1-200) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ELP3 |
| Gene ID | 55140 |
| Uniprot ID | ELP3_HUMAN |
| Immunogen Region | 1-200 |
| Immunogen Sequence | MRQKRKGDLSPAELMMLTIG DVIKQLIEAHEQGKDIDLNK VKTKTAAKYGLSAQPRLVDI IAAVPPQYRKVLMPKLKAKP IRTASGIAVVAVMCKPHRCP HISFTGNICVYCPGGPDSDF EYSTQSYTGYEPTSMRAIRA RYDPFLQTRHRIEQLKQLGH SVDKVEFIVMGGTFMALPEE YRDYFIRNLHDALSGHTSNN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human KAT9/Elp3 (NP_060561.3). |
Additional Info
| Tissue Specificity | Expressed in the cerebellum and spinal motor neurons. |
| Post Translational Modifications | Tyrosine-phosphorylated.phosphorylation on Tyr-202 does not affect elongator complex integrity or ELP3 protein stability. Also serine/threonine-phosphorylated. |
| Function | Catalytic tRNA acetyltransferase subunit of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. May also act as a protein lysine acetyltransferase by mediating acetylation of target proteins.such activity is however unclear in vivo and recent evidences suggest that ELP3 primarily acts as a tRNA acetyltransferase. Involved in neurogenesis: regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation. Required for acetylation of GJA1 in the developing cerebral cortex. |
| Protein Name | Elongator Complex Protein 3Help3Trna Uridine(34 Acetyltransferase |
| Database Links | Reactome: R-HSA-3214847 |
| Cellular Localisation | CytoplasmNucleusIsoform 1: NucleusIsoform 2: Cytoplasm |
| Alternative Antibody Names | Anti-Elongator Complex Protein 3 antibodyAnti-Help3 antibodyAnti-Trna Uridine(34 Acetyltransferase antibodyAnti-ELP3 antibody |
Information sourced from Uniprot.org