• Immunoprecipitation analysis of 200 Mu g extracts of MCF7 cells using 1 Mu g NAA20 antibody (STJ29185). Western blot was performed from the immunoprecipitate using NAA20 antibody (STJ29185) at a dilution of 1:1000.
  • Immunohistochemistry analysis of NAA20 in paraffin-embedded human oophoroma using NAA20 Rabbit polyclonal antibody (STJ29185) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of NAA20 in paraffin-embedded rat kidney using NAA20 Rabbit polyclonal antibody (STJ29185) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using NAA20 Rabbit polyclonal antibody (STJ29185) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-NAA20 antibody (49-178) (STJ29185)

SKU:
STJ29185

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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NAA20 (49-178) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NAA20
Gene ID: 51126
Uniprot ID: NAA20_HUMAN
Immunogen Region: 49-178
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 49-178 of human NAA20 (NP_057184.1).
Immunogen Sequence: PGGELMGYIMGKAEGSVARE EWHGHVTALSVAPEFRRLGL AAKLMELLEEISERKGGFFV DLFVRVSNQVAVNMYKQLGY SVYRTVIEYYSASNGEPDED AYDMRKALSRDTEKKSIIPL PHPVRPEDIE
Function Catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp, Met-Glu, Met-Asn and Met-Gln. Proteins with cell cycle functions are overrepresented in the pool of NatB substrates. Required for maintaining the structure and function of actomyosin fibers and for proper cellular migration.
Protein Name N-Alpha-Acetyltransferase 20
Methionine N-Acetyltransferase
N-Acetyltransferase 5
N-Terminal Acetyltransferase B Complex Catalytic Subunit Naa20
N-Terminal Acetyltransferase B Complex Catalytic Subunit Nat5
Natb Complex Subunit Nat5
Natb Catalytic Subunit
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-N-Alpha-Acetyltransferase 20 antibody
Anti-Methionine N-Acetyltransferase antibody
Anti-N-Acetyltransferase 5 antibody
Anti-N-Terminal Acetyltransferase B Complex Catalytic Subunit Naa20 antibody
Anti-N-Terminal Acetyltransferase B Complex Catalytic Subunit Nat5 antibody
Anti-Natb Complex Subunit Nat5 antibody
Anti-Natb Catalytic Subunit antibody
Anti-NAA20 antibody
Anti-NAT5 antibody

Information sourced from Uniprot.org

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