Anti-N6AMT1 antibody (1-214) (STJ11104540)
SPECIFICATIONS
ClonalityPolyclonal
Hostrabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Methyltransferase N6amt1 (1-214) antibody for use in WB, IHC-P and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HEMK2 |
| Gene ID | 29104 |
| Uniprot ID | HEMK2_HUMAN |
| Immunogen Region | 1-214 |
| Immunogen Sequence | MAGENFATPFHGHVGRGAFS DVYEPAEDTFLLLDALEAAA AELAGVEICLEVGSGSGVVS AFLASMIGPQALYMCTDINP EAAACTLETARCNKVHIQPV ITDLVKGLLPRLTEKVDLLV FNPPYVVTPPQEVGSHGIEA AWAGGRNGREVMDRFFPLVP DLLSPRGLFYLVTIKENNPE EILKIMKTKGLQGTTALSRQ AGQETLSVLKFTKS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-214 of human N6AMT1/HEMK2 (NP_037372.4). |
Additional Info
| Tissue Specificity | Widely expressed, with highest expression in parathyroid and pituitary glands, followed by adrenal gland and kidney, and lowest expression in leukocytes and mammary gland. |
| Post Translational Modifications | Isoform 1: Ubiquitinated, leading to its degradation by the proteasome. Isoform 2: Ubiquitinated, leading to its degradation by the proteasome. |
| Function | Methyltransferase that can methylate proteins and, to a lower extent, arsenic. Catalytic subunit of a heterodimer with TRMT112, which monomethylates 'Lys-12' of histone H4 (H4K12me1), a modification present at the promoters of numerous genes encoding cell cycle regulators. Catalytic subunit of a heterodimer with TRMT112, which catalyzes N5-methylation of Glu residue of proteins with a Gly-Gln-Xaa-Xaa-Xaa-Arg motif. Methylates ETF1 on 'Gln-185'.ETF1 needs to be complexed to ERF3 in its GTP-bound form to be efficiently methylated. May also play a role in the modulation of arsenic-induced toxicity by mediating the conversion of monomethylarsonous acid (3+) into the less toxic dimethylarsonic acid. It however only plays a limited role in arsenic metabolism compared with AS3MT. |
| Protein Name | Methyltransferase Hemk2Hemk Methyltransferase Family Member 2M.hsahemk2pLysine N-Methyltransferase 9Methylarsonite Methyltransferase N6amt1Methyltransferase N6amt1Protein N(5-Glutamine Methyltransferase |
| Database Links | Reactome: R-HSA-156581Reactome: R-HSA-72764 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Methyltransferase Hemk2 antibodyAnti-Hemk Methyltransferase Family Member 2 antibodyAnti-M.hsahemk2p antibodyAnti-Lysine N-Methyltransferase 9 antibodyAnti-Methylarsonite Methyltransferase N6amt1 antibodyAnti-Methyltransferase N6amt1 antibodyAnti-Protein N(5-Glutamine Methyltransferase antibodyAnti-HEMK2 antibodyAnti-C21orf127 antibodyAnti-KMT9 antibodyAnti-N6AMT1 antibodyAnti-PRED28 antibody |
Information sourced from Uniprot.org