| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-N4BP1 (190-893) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
N4BP1 |
| Gene ID: |
9683 |
| Uniprot ID: |
N4BP1_HUMAN |
| Immunogen Region: |
190-893 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 190-893 of mouse N4BP1 (NP_085040.2). |
| Immunogen Sequence: |
TQGEESLFETDDDVITVGDV RPPEYTQSAATGPSSARDEV VVQEDSRNKARTPVSELTKH MDTVFSSSPDVLFVPVNGLS PDEDALSKDRVCHKRRSSDT EERHTKKQFSLENVPEGELL PDGKGSAGNEVIDLSDPASN STNLSPDGKDTTEEMEYNIL VNFFKTMGYSQEIVEKVIRE YGPSTEPLLLLEEIEKENKR LQEDRDFPPCTVYPDASQSR NAGVGSTTNELTADSTPKK |
| Tissue Specificity | Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary. |
| Post Translational Modifications | Proteolytically cleaved by CASP8 downstream of TLR3 or TLR4, leading to its inactivation. Mainly cleaved at Asp-490 by CASP8. Cleaved by caspase-like protein MALT1 in T-cells following TCR-mediated activation, leading to its inactivation and subsequent viral reactivation during HIV-1 infection. Mono- and polyubiquitinated on the CoCUN region. Monoubiquitinated by NEDD4. Polyubiquitinated, leading to its degradation by the proteasome. Sumoylated with SUMO1, abrogating polyubiquitination and subsequent degradation. Desumoylated by SENP1, leading to accumulation in PML nuclear bodies. |
| Function | Potent suppressor of cytokine production that acts as a regulator of innate immune signaling and inflammation. Acts as a key negative regulator of select cytokine and chemokine responses elicited by TRIF-independent Toll-like receptors (TLRs), thereby limiting inflammatory cytokine responses to minor insults. In response to more threatening pathogens, cleaved by CASP8 downstream of TLR3 or TLR4, leading to its inactivation, thereby allowing production of inflammatory cytokines. Acts as a restriction factor against some viruses, such as HIV-1: restricts HIV-1 replication by binding to HIV-1 mRNAs and mediating their degradation via its ribonuclease activity. Also acts as an inhibitor of the E3 ubiquitin-protein ligase ITCH: acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates. |
| Protein Name | Nedd4-Binding Protein 1N4bp1 |
| Database Links | Reactome: R-HSA-9758274 |
| Cellular Localisation | CytoplasmCytosolNucleusNucleolusPml BodyPrimarily Localizes To The NucleolusAlso Localizes To The Pml Nuclear BodiesWhen Desumoylated |
| Alternative Antibody Names | Anti-Nedd4-Binding Protein 1 antibodyAnti-N4bp1 antibodyAnti-N4BP1 antibodyAnti-KIAA0615 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
