Anti-N4BP1 antibody (190-893) (STJ110772)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal N4BP1 (190-893) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | N4BP1 |
| Gene ID | 9683 |
| Uniprot ID | N4BP1_HUMAN |
| Immunogen Region | 190-893 |
| Immunogen Sequence | TQGEESLFETDDDVITVGDV RPPEYTQSAATGPSSARDEV VVQEDSRNKARTPVSELTKH MDTVFSSSPDVLFVPVNGLS PDEDALSKDRVCHKRRSSDT EERHTKKQFSLENVPEGELL PDGKGSAGNEVIDLSDPASN STNLSPDGKDTTEEMEYNIL VNFFKTMGYSQEIVEKVIRE YGPSTEPLLLLEEIEKENKR LQEDRDFPPCTVYPDASQSR NAGVGSTTNELTADSTPKK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 190-590 of mouse N4BP1 (NP_085040.2). |
Additional Info
| Tissue Specificity | Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary. |
| Post Translational Modifications | Proteolytically cleaved by CASP8 downstream of TLR3 or TLR4, leading to its inactivation. Mainly cleaved at Asp-490 by CASP8. Cleaved by caspase-like protein MALT1 in T-cells following TCR-mediated activation, leading to its inactivation and subsequent viral reactivation during HIV-1 infection. Mono- and polyubiquitinated on the CoCUN region. Monoubiquitinated by NEDD4. Polyubiquitinated, leading to its degradation by the proteasome. Sumoylated with SUMO1, abrogating polyubiquitination and subsequent degradation. Desumoylated by SENP1, leading to accumulation in PML nuclear bodies. |
| Function | Potent suppressor of cytokine production that acts as a regulator of innate immune signaling and inflammation. Acts as a key negative regulator of select cytokine and chemokine responses elicited by TRIF-independent Toll-like receptors (TLRs), thereby limiting inflammatory cytokine responses to minor insults. In response to more threatening pathogens, cleaved by CASP8 downstream of TLR3 or TLR4, leading to its inactivation, thereby allowing production of inflammatory cytokines. Acts as a restriction factor against some viruses, such as HIV-1: restricts HIV-1 replication by binding to HIV-1 mRNAs and mediating their degradation via its ribonuclease activity. Also acts as an inhibitor of the E3 ubiquitin-protein ligase ITCH: acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates. |
| Protein Name | Nedd4-Binding Protein 1N4bp1 |
| Database Links | Reactome: R-HSA-9758274 |
| Cellular Localisation | CytoplasmCytosolNucleusNucleolusPml BodyPrimarily Localizes To The NucleolusAlso Localizes To The Pml Nuclear BodiesWhen Desumoylated |
| Alternative Antibody Names | Anti-Nedd4-Binding Protein 1 antibodyAnti-N4bp1 antibodyAnti-N4BP1 antibodyAnti-KIAA0615 antibody |
Information sourced from Uniprot.org