Anti-Myosin IXB antibody (Internal) (STJ70933)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-Myosin IXB (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 200kDa band observed in Human Liver lysates (calculated MW of 243kDa according to NP_004136.2). Recommended concentration: 2-6µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | MYO9B |
| Gene ID | 4650 |
| Uniprot ID | MYO9B_HUMAN |
| Accession Number | NP_004136.2; NP_001123537.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | KNDFEPVKQSKAQ |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_004136.2; NP_001123537.1). |
Additional Info
| Function | Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Binds actin with high affinity both in the absence and presence of ATP and its mechanochemical activity is inhibited by calcium ions. Also acts as a GTPase activator for RHOA. Plays a role in the regulation of cell migration via its role as RHOA GTPase activator. This is regulated by its interaction with the SLIT2 receptor ROBO1.interaction with ROBO1 impairs interaction with RHOA and subsequent activation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA. |
| Protein Name | Unconventional Myosin-IxbUnconventional Myosin-9b |
| Database Links | Reactome: R-HSA-2029482Reactome: R-HSA-8980692Reactome: R-HSA-8985586Reactome: R-HSA-9013026Reactome: R-HSA-9013106Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9035034Reactome: R-HSA-9664422 |
| Cellular Localisation | CytoplasmCell CortexPerinuclear RegionCytoskeletonIn Undifferentiated Cells Colocalizes With F-Actin In The Cell Periphery While In Differentiated Cells Its Localization Is Cytoplasmic With The Highest Levels In The Perinuclear Region |
| Alternative Antibody Names | Anti-Unconventional Myosin-Ixb antibodyAnti-Unconventional Myosin-9b antibodyAnti-MYO9B antibodyAnti-MYR5 antibody |
Information sourced from Uniprot.org