| Host: |
Mouse |
| Applications: |
IHC-F/IHC-P/IF/WB |
| Reactivity: |
Chicken/Human/Mouse/Rabbit |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Myosin is suitable for use in Immunohistochemistry, Immunofluorescence and Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
414 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgM |
| Formulation: |
Contains 0.09% sodium azide |
| Dilution Range: |
IF 1:10IHC 1:5-1:10 |
| Storage Instruction: |
Store at 2-8°C upon receipt. |
| Immunogen: |
Chicken skeletal muscle cell preparation |
| Background | Mab 414 reacts with myosin-containing elements in most cell types. Antigen localization: cytoplasm In Western blot experiments the antibody reacts with a 75kD part of the heavy chain (epitope location on a myosin-common site-chain) , no reaction with light chains.
It is not reactive with alpha-cardiac myosin. Positive control: muscle, myofibrils (chicken) |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and IDH1 knockout (KO) HeLa cells, using [KO Validated] IDH1 Rabbit polyclonal antibody (STJ119996) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/98781/382830/STJ119996_1__99034.1713151333.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and IDH1 knockout (KO) HeLa cells, using [KO Validated] IDH1 Rabbit polyclonal antibody (STJ119996) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")