• Western blot analysis of lysates from wild type (WT) and MYOF knockout (KO) HeLa (KO) cells, using [KO Validated] MYOF Rabbit polyclonal antibody (STJ117622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MYOF antibody (1-190) (STJ117622)

SKU:
STJ117622

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MYOF (1-190) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYOF
Gene ID: 26509
Uniprot ID: MYOF_HUMAN
Immunogen Region: 1-190
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human MYOF (NP_038479.1).
Immunogen Sequence: MLRVIVESASNIPKTKFGKP DPIVSVIFKDEKKKTKKVDN ELNPVWNEILEFDLRGIPLD FSSSLGIIVKDFETIGQNKL IGTATVALKDLTGDQSRSLP YKLISLLNEKGQDTGATIDL VIGYDPPSAPHPNDLSGPSV PGMGGDGEEDEGDEDRLDNA VRGPGPKGPVGTVSEAQLAR RLTKVKNSRR
Tissue Specificity Expressed in myoblast and endothelial cells (at protein level). Highly expressed in cardiac and skeletal muscles. Also present in lung, and at very low levels in kidney, placenta and brain.
Function Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR.
Protein Name Myoferlin
Fer-1-Like Protein 3
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Nucleus Membrane
Cytoplasmic Vesicle Membrane
Concentrated At The Membrane Sites Of Both Myoblast-Myoblast And Myoblast-Myotube Fusions
Detected At The Plasmalemma In Endothelial Cells Lining Intact Blood Vessels
Found At Nuclear And Plasma Membranes
Enriched In Undifferentiated Myoblasts Near The Plasma Membrane In Puncate Structures
Alternative Antibody Names Anti-Myoferlin antibody
Anti-Fer-1-Like Protein 3 antibody
Anti-MYOF antibody
Anti-FER1L3 antibody
Anti-KIAA1207 antibody

Information sourced from Uniprot.org

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