• Western blot analysis of extracts of various cell lines, using MYO5A antibody (STJ111872) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of various cell lines, using MYO5A antibody (STJ111872) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MYO5A antibody (950-1150) (STJ111872)

SKU:
STJ111872

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MYO5A (950-1150) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYO5A
Gene ID: 4644
Uniprot ID: MYO5A_HUMAN
Immunogen Region: 950-1150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 950-1150 of human MYO5A (NP_000250.3).
Immunogen Sequence: LTNLEGIYNSETEKLRSDLE RLQLSEEEAKVATGRVLSLQ EEIAKLRKDLEQTRSEKKCI EEHADRYKQETEQLVSNLKE ENTLLKQEKEALNHRIVQQA KEMTETMEKKLVEETKQLEL DLNDERLRYQNLLNEFSRLE ERYDDLKEEMTLMVHVPKPG HKRTDSTHSSNESEYIFSSE IAEMEDIPSRTEEPSEKKVP L
Tissue Specificity Detected in melanocytes.
Function Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.
Protein Name Unconventional Myosin-Va
Dilute Myosin Heavy Chain - Non-Muscle
Myosin Heavy Chain 12
Myosin-12
Myoxin
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-2029482
Reactome: R-HSA-264876
Reactome: R-HSA-9664422
Alternative Antibody Names Anti-Unconventional Myosin-Va antibody
Anti-Dilute Myosin Heavy Chain - Non-Muscle antibody
Anti-Myosin Heavy Chain 12 antibody
Anti-Myosin-12 antibody
Anti-Myoxin antibody
Anti-MYO5A antibody
Anti-MYH12 antibody

Information sourced from Uniprot.org

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