• Western blot analysis of lysates from HeLa and Jurkat cells, using MYLIP Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of Jurkat cells using MYLIP Polyclonal Antibody
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MYLIP Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using MYLIP Polyclonal Antibody

Anti-MYLIP antibody (161-210) (STJ94304)

SKU:
STJ94304

$62.10 - $305.10
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-E3 ubiquitin-protein ligase MYLIP (161-210) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYLIP
Gene ID: 29116
Uniprot ID: MYLIP_HUMAN
Immunogen Region: 161-210
Specificity: MYLIP Polyclonal Antibody detects endogenous levels of MYLIP protein.
Immunogen: The antiserum was produced against synthesized peptide derived from human MYLIP. AA range:161-210
Tissue Specificity Ubiquitously expressed.
Post Translational Modifications Autoubiquitinated.
Function E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.
Protein Name E3 Ubiquitin-Protein Ligase Mylip
Inducible Degrader Of The Ldl-Receptor
Idol
Myosin Regulatory Light Chain Interacting Protein
Mir
Ring-Type E3 Ubiquitin Transferase Mylip
Database Links Reactome: R-HSA-8866427
Reactome: R-HSA-9031525
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Mylip antibody
Anti-Inducible Degrader Of The Ldl-Receptor antibody
Anti-Idol antibody
Anti-Myosin Regulatory Light Chain Interacting Protein antibody
Anti-Mir antibody
Anti-Ring-Type E3 Ubiquitin Transferase Mylip antibody
Anti-MYLIP antibody
Anti-BZF1 antibody
Anti-IDOL antibody
Anti-BM-023 antibody
Anti-PP5242 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance