• Immunofluorescence analysis of human placenta cells using SMMHC/SMMHC/MYH11 antibody (STJ112723) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using SMMHC/MYH11 antibody (STJ112723) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MYH11 antibody (1680-1979) (STJ112723)

SKU:
STJ112723

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MYH11 (1680-1979) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYH11
Gene ID: 4629
Uniprot ID: MYH11_HUMAN
Immunogen Region: 1680-1979
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1680-1979 of human SMMHC/SMMHC/MYH11 (NP_001035203.1).
Immunogen Sequence: ATAKENEKKAKSLEADLMQL QEDLAAAERARKQADLEKEE LAEELASSLSGRNALQDEKR RLEARIAQLEEELEEEQGNM EAMSDRVRKATQQAEQLSNE LATERSTAQKNESARQQLER QNKELRSKLHEMEGAVKSKF KSTIAALEAKIAQLEEQVEQ EAREKQAATKSLKQKDKKLK EILLQVEDERKMAEQYKEQA EKGNARVKQLKRQLEEAEEE SQRINANRRKLQRELDEAT
Tissue Specificity Smooth muscle.expressed in the umbilical artery, bladder, esophagus and trachea. Isoform 1 is mostly found in slowly contracting tonic muscles.
Function Muscle contraction.
Protein Name Myosin-11
Myosin Heavy Chain 11
Myosin Heavy Chain - Smooth Muscle Isoform
Smmhc
Database Links Reactome: R-HSA-3928663
Reactome: R-HSA-416572
Reactome: R-HSA-445355
Reactome: R-HSA-5625740
Reactome: R-HSA-5625900
Reactome: R-HSA-5627117
Reactome: R-HSA-5627123
Cellular Localisation Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Thick Filaments Of The Myofibrils
Alternative Antibody Names Anti-Myosin-11 antibody
Anti-Myosin Heavy Chain 11 antibody
Anti-Myosin Heavy Chain - Smooth Muscle Isoform antibody
Anti-Smmhc antibody
Anti-MYH11 antibody
Anti-KIAA0866 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance