• Western blot analysis of extracts of HL-60 cells, using Myeloperoxidase (MPO) antibody (STJ11102741) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Myeloperoxidase (MPO) rabbit monoclonal antibody (STJ11102741) at dilution of 1:1000 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human extranodal NK-T cell lymphoma using Myeloperoxidase (MPO) rabbit monoclonal antibody (STJ11102741) at dilution of 1:1000 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human hepatocholangiocarcinoma using Myeloperoxidase (MPO) rabbit monoclonal antibody (STJ11102741) at dilution of 1:1000 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver using Myeloperoxidase (MPO) rabbit monoclonal antibody (STJ11102741) at dilution of 1:1000 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using Myeloperoxidase (MPO) rabbit monoclonal antibody (STJ11102741) at dilution of 1:1000 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-Myeloperoxidase antibody (646-745) [S1MR] (STJ11102741)

SKU:
STJ11102741

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Myeloperoxidase (MPO) (646-745) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MPO
Gene ID: 4353
Uniprot ID: PERM_HUMAN
Immunogen Region: 646-745
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 646-745 of human Myeloperoxidase (MPO) (NP_000241.1).
Immunogen Sequence: GVSEPLKRKGRVGPLLACII GTQFRKLRDGDRFWWENEGV FSMQQRQALAQISLPRIICD NTGITTVSKNNIFMSNSYPR DFVNCSTLPALNLASWREAS
Function Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage.
Protein Name Myeloperoxidase
Mpo Cleaved Into - Myeloperoxidase - 89 Kda Myeloperoxidase - 84 Kda Myeloperoxidase - Myeloperoxidase Light Chain - Myeloperoxidase Heavy Chain
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-8941413
Cellular Localisation Lysosome
Alternative Antibody Names Anti-Myeloperoxidase antibody
Anti-Mpo Cleaved Into - Myeloperoxidase - 89 Kda Myeloperoxidase - 84 Kda Myeloperoxidase - Myeloperoxidase Light Chain - Myeloperoxidase Heavy Chain antibody
Anti-MPO antibody

Information sourced from Uniprot.org

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