Anti-Myelin basic protein antibody (STJA0040650)
SPECIFICATIONS
ClonalityPolyclonal
HostChicken
ConjugationUnconjugated
IsotypeIgY
ImmunogenThree peptide sequences conserved in higher verterbrate MBP protein.
General Information
| Short Description | Chicken polyclonal anti-Myelin basic protein for use in ICC, IHC-F, IHC-P and WB in Bovine, Human, Mouse, Other Mammals and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ICC/IHC-F/IHC-P/WB |
| Host | Chicken |
| Reactivity | Bovine/Human/Mouse/Other Mammals/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgY |
| Conjugation | Unconjugated |
| Purification | Affinity purified IgY preparation |
| Dilution Range | WB 1:5000-1:10000IHC 1:500-1:1000ICC 1:500-1:1000 |
| Formulation | Lyophilized IgY preparation, with sodium azide. |
| Storage Instruction | Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at-20°C for a higher stability. Avoid freeze-tha |
Target Information
| Gene Symbol | MBP |
| Gene ID | 4155 |
| Uniprot ID | MBP_HUMAN |
| Immunogen | Three peptide sequences conserved in higher verterbrate MBP protein. |
| Immunogen Sequence | Human |
| Specificity | The specificity of this antibody has been confirmed by WB. This antibody stains a prominent band at approx. 20 kDa. A suitable control tissue is rat spinal cord or peripheral nerve homogenate. The major isoforms of MBP run as a closely spaced double |
Additional Info
| Tissue Specificity | MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. |
| Post Translational Modifications | Several charge isomers of MBP.C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form).are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic. The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6). Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated. Proteolytically cleaved in B cell lysosomes by cathepsin CTSG which degrades the major immunogenic MBP epitope and prevents the activation of MBP-specific autoreactive T cells. Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1. |
| Function | The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. |
| Protein Name | Myelin Basic ProteinMbpMyelin A1 ProteinMyelin Membrane Encephalitogenic Protein |
| Database Links | Reactome: R-HSA-9619665 |
| Cellular Localisation | Myelin MembranePeripheral Membrane ProteinCytoplasmic SideCytoplasmic Side Of MyelinIsoform 3: NucleusTargeted To Nucleus In Oligodendrocytes |
| Alternative Antibody Names | Anti-Myelin Basic Protein antibodyAnti-Mbp antibodyAnti-Myelin A1 Protein antibodyAnti-Myelin Membrane Encephalitogenic Protein antibodyAnti-MBP antibody |
Information sourced from Uniprot.org