• Immunofluorescence analysis of U2OS cells using MyD88 Rabbit polyclonal antibody (STJ24659) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using MyD88 Rabbit polyclonal antibody (STJ24659) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MYD88 antibody (20-140) (STJ24659)

SKU:
STJ24659

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MyD88 (20-140) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYD88
Gene ID: 4615
Uniprot ID: MYD88_HUMAN
Immunogen Region: 20-140
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 20-140 of human MyD88 (NP_002459.2).
Immunogen Sequence: GAGSAAPVSSTSSLPLAALN MRVRRRLSLFLNVRTQVAAD WTALAEEMDFEYLEIRQLET QADPTGRLLDAWQGRPGASV GRLLELLTKLGRDDVLLELG PSIEEDCQKYILKQQQEEAE K
Tissue Specificity Ubiquitous.
Post Translational Modifications Ubiquitinated.undergoes 'Lys-63'-linked polyubiquitination. OTUD4 specifically hydrolyzes 'Lys-63'-linked polyubiquitinated MYD88.
Function Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Upon TLR8 activation by GU-rich single-stranded RNA (GU-rich RNA) derived from viruses such as SARS-CoV-2, SARS-CoV and HIV-1, induces IL1B release through NLRP3 inflammasome activation. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine.
Protein Name Myeloid Differentiation Primary Response Protein Myd88
Database Links Reactome: R-HSA-1236974
Reactome: R-HSA-1257604
Reactome: R-HSA-166058
Reactome: R-HSA-1810476
Reactome: R-HSA-209543
Reactome: R-HSA-3134963
Reactome: R-HSA-5602498
Reactome: R-HSA-5602680
Reactome: R-HSA-5603037
Reactome: R-HSA-5603041
Reactome: R-HSA-6811558
Reactome: R-HSA-9020702
Reactome: R-HSA-975110
Reactome: R-HSA-975138
Reactome: R-HSA-975155
Reactome: R-HSA-975871
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Myeloid Differentiation Primary Response Protein Myd88 antibody
Anti-MYD88 antibody

Information sourced from Uniprot.org

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