Anti-MYCBP2 antibody (STJA0008519)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human MYCBP2
General Information
| Short Description | Rabbit polyclonal anti-E3 ubiquitin-protein ligase MYCBP2 for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MYCBP2 |
| Gene ID | 23077 |
| Uniprot ID | MYCB2_HUMAN |
| Immunogen | Synthesized peptide derived from human MYCBP2 |
| Specificity | This antibody detects endogenous levels of MYCBP2 at Human, Mouse |
Additional Info
| Post Translational Modifications | Autoubiquitinated. |
| Function | Atypical E3 ubiquitin-protein ligase which specifically mediates ubiquitination of threonine and serine residues on target proteins, instead of ubiquitinating lysine residues. Shows esterification activity towards both threonine and serine, with a preference for threonine, and acts via two essential catalytic cysteine residues that relay ubiquitin to its substrate via thioester intermediates. Interacts with the E2 enzymes UBE2D1, UBE2D3, UBE2E1 and UBE2L3. Plays a key role in neural development, probably by mediating ubiquitination of threonine residues on target proteins (Probable). Involved in different processes such as regulation of neurite outgrowth, synaptic growth, synaptogenesis and axon degeneration. Required for the formation of major central nervous system axon tracts. Required for proper axon growth by regulating axon navigation and axon branching: acts by regulating the subcellular location and stability of MAP3K12/DLK. Required for proper localization of retinogeniculate projections but not for eye-specific segregation. Regulates axon guidance in the olfactory system. Involved in Wallerian axon degeneration, an evolutionarily conserved process that drives the loss of damaged axons: acts by promoting destabilization of NMNAT2, probably via ubiquitination of NMNAT2. Catalyzes ubiquitination of threonine and/or serine residues on NMNAT2, consequences of threonine and/or serine ubiquitination are however unknown. Regulates the internalization of TRPV1 in peripheral sensory neurons. Mediates ubiquitination and subsequent proteasomal degradation of TSC2/tuberin. Independently of the E3 ubiquitin-protein ligase activity, also acts as a guanosine exchange factor (GEF) for RAN in neurons of dorsal root ganglia. May function as a facilitator or regulator of transcriptional activation by MYC. Acts in concert with HUWE1 to regulate the circadian clock gene expression by promoting the lithium-induced ubiquination and degradation of NR1D1. |
| Protein Name | E3 Ubiquitin-Protein Ligase Mycbp2Myc-Binding Protein 2Protein Associated With Myc |
| Database Links | |
| Cellular Localisation | NucleusCell ProjectionAxonCytoplasmCytoskeletonLocalizes To Axon Shafts And Associates With Microtubule CytoskeletonTranslocates To The Nucleus Following Interaction With Sumoylated Rangap1 |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Mycbp2 antibodyAnti-Myc-Binding Protein 2 antibodyAnti-Protein Associated With Myc antibodyAnti-MYCBP2 antibodyAnti-KIAA0916 antibodyAnti-PAM antibody |
Information sourced from Uniprot.org