Anti-MURF2/TRIM55 antibody (C-Term) (STJ70333)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-MURF2/TRIM55 (C-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications. |
| Applications | Pep-ELISA/WB/IHC/IF/FC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Pig |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 58kDa band observed in lysates of cell line HeLa (calculated MW of 60.5kDa according to NP_908973.1). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temp |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | TRIM55 |
| Gene ID | 84675 |
| Uniprot ID | TRI55_HUMAN |
| Accession Number | NP_908973.1; NP_908974.1; NP_908975.1 |
| Immunogen Region | C-Term |
| Immunogen Sequence | HIFSFSWLNSLNE |
| Specificity | This antibody is expected to recognize isoforms 1, 3 and 4 (NP_908973.1, NP_908974.1and NP_908975.1 resp.). |
Additional Info
| Post Translational Modifications | Targeted for degradation through the proteasomal and lysosomal pathways in the presence of SUMO3. |
| Function | E3 ubiquitin ligase that plays an important role in regulating cardiac development and contractility, muscle growth, metabolism, and fiber-type differentiation. Acts as a critical factor that regulates cardiomyocyte size during development in concert with TRIM63 by regulating E2F1-mediated gene expression. Plays a role in apoptosis induction in cardiomyocytes by promoting ubiquitination of the DUSP1 phosphatase. Promotes non-canonical NF-kappa-B signaling and B-cell-mediated immune responses by mediating NFKB2 'Lys-48'-linked ubiquitination and processing. In turn, NFKB2 is further processed by valosin-containing protein/VCP, an ATPase that mediates ubiquitin-dependent protein degradation by the proteasome. May play a role in preventing macrophages from producing inflammatory factors and migrating by downregulating the level of nuclear NF-kappa-B subunit RELA. Modifies also PPARG via polyubiquitination and accelerates PPARG proteasomal degradation to inhibit its activity. |
| Protein Name | Tripartite Motif-Containing Protein 55Muscle-Specific Ring Finger Protein 2Murf-2Murf2Ring Finger Protein 29 |
| Cellular Localisation | NucleusCytoplasmTlr4 Signaling Pathway Promotes Nuclear Translocation |
| Alternative Antibody Names | Anti-Tripartite Motif-Containing Protein 55 antibodyAnti-Muscle-Specific Ring Finger Protein 2 antibodyAnti-Murf-2 antibodyAnti-Murf2 antibodyAnti-Ring Finger Protein 29 antibodyAnti-TRIM55 antibodyAnti-MURF2 antibodyAnti-RNF29 antibody |
Information sourced from Uniprot.org