| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Mouse/Rat/Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Mucolipin 1 is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB. A dilution of 1: 1000 to 1:3000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
MCOLN1 |
| Gene ID: |
57192 |
| Uniprot ID: |
MCLN1_HUMAN |
| Specificity: |
Specific for Mucolipin 1, isoforms 1 and 2. |
| Immunogen: |
A synthetic peptide from mouse Mucolipin 1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in human, monkey and rat. |
| Post Translational Modifications | Palmitoylated.involved in association with membranes. Phosphorylation by PKA inhibits channel activity. Dephosphorylation increases activity. Proteolytically cleaved probably involving multiple lysosomal proteases including cathepsin B.inhibits lysosomal channel activity. |
| Function | Nonselective cation channel probably playing a role in the regulation of membrane trafficking events and of metal homeostasis. Acts as a Ca(2+)-permeable cation channel with inwardly rectifying activity. Proposed to play a major role in Ca(2+) release from late endosome and lysosome vesicles to the cytoplasm, which is important for many lysosome-dependent cellular events, including the fusion and trafficking of these organelles, exocytosis and autophagy. Required for efficient uptake of large particles in macrophages in which Ca(2+) release from the lysosomes triggers lysosomal exocytosis. May also play a role in phagosome-lysosome fusion. Involved in lactosylceramide trafficking indicative for a role in the regulation of late endocytic membrane fusion/fission events. By mediating lysosomal Ca(2+) release is involved in regulation of mTORC1 signaling and in mTOR/TFEB-dependent lysosomal adaptation to environmental cues such as nutrient levels. Seems to act as lysosomal active oxygen species (ROS) sensor involved in ROS-induced TFEB activation and autophagy. Also functions as a Fe(2+) permeable channel in late endosomes and lysosomes. Also permeable to Mg(2+), Na(+). K(+) and Cs(+). Proposed to play a role in zinc homeostasis probably implicating its association with TMEM163 In adaptive immunity, TRPML2 and TRPML1 may play redundant roles in the function of the specialized lysosomes of B cells. May contribute to cellular lipase activity within the late endosomal pathway or at the cell surface which may be involved in processes of membrane reshaping and vesiculation, especially the growth of tubular structures. However, it is not known, whether it conveys the enzymatic activity directly, or merely facilitates the activity of an associated phospholipase. |
| Protein Name | Mucolipin-1Ml1Mg-2MucolipidinTransient Receptor Potential Channel Mucolipin 1Trpml1 |
| Database Links | Reactome: R-HSA-3295583Reactome: R-HSA-917977 |
| Cellular Localisation | Late Endosome MembraneMulti-Pass Membrane ProteinLysosome MembraneCytoplasmic Vesicle MembraneCell ProjectionPhagocytic CupCytoplasmic VesiclePhagosome MembraneCell MembraneDelivery From The Trans-Golgi To Lysosomes Seems To Occur Mainly In A Direct Intracellular Manner Without Intermediate Delivery To The Plasma MembraneUnder Normal ConditionsRestricted To Intracellular Compartments So That Only A Very Minor Proportion Is Present At The Cell Membrane |
| Alternative Antibody Names | Anti-Mucolipin-1 antibodyAnti-Ml1 antibodyAnti-Mg-2 antibodyAnti-Mucolipidin antibodyAnti-Transient Receptor Potential Channel Mucolipin 1 antibodyAnti-Trpml1 antibodyAnti-MCOLN1 antibodyAnti-ML4 antibodyAnti-TRPML1 antibodyAnti-MSTP080 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
