• Western blot analysis of extracts of Mouse stomach, using Mucin 5AC (MUC5AC) antibody (STJ119455) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-MUC5AC antibody (1550-1900) (STJ119455)

SKU:
STJ119455

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MUC5A (1550-1900) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MUC5AC
Gene ID: 4586
Uniprot ID: MUC5A_HUMAN
Immunogen Region: 1550-1900
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1550-1900 of human Mucin 5AC (MUC5AC) (NP_001291288.1).
Immunogen Sequence: TSSMSTTAPGTSVVSSKPTP TEPSTSSCLQELCTWTEWID GSYPAPGINGGDFDTFQNLR DEGYTFCESPRSVQCRAESF PNTPLADLGQDVICSHTEGL ICLNKNQLPPICYNYEIRIQ CCETVNVCRDITRLPKTVAT TRPTPHPTGAQTQTTFTTHM PSASTEQPTATSRGGPTATS VTQGTHTTLVTRNCHPRCTW TKWFDVDFPSPGPHGGDKET YNNIIRSGEKICRRPEEIT
Tissue Specificity Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum.
Post Translational Modifications C-, O- and N-glycosylated. O-glycosylated on the second and last Thr of the Thr-/Ser-rich tandem repeats TTPSPVPTTSTTSA. One form of glycosylation is also known as Lewis B (LeB) blood group antigen, a tetrasaccharide consisting of N-acetylglucosamine having a fucosyl residue attached. It has a role as an epitope and antigen and functions as a receptor for H.pylori binding and facilitates infection. C-mannosylation in the Cys-rich subdomains may be required for proper folding of these regions and for export from the endoplasmic reticulum during biosynthesis. Proteolytic cleavage in the C-terminal is initiated early in the secretory pathway and does not involve a serine protease. The extent of cleavage is increased in the acidic parts of the secretory pathway. Cleavage generates a reactive group which could link the protein to a primary amide.
Function Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system. Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD).
Protein Name Mucin-5ac
Muc-5ac
Gastric Mucin
Major Airway Glycoprotein
Mucin-5 Subtype Ac - Tracheobronchial
Tracheobronchial Mucin
Tbm
Database Links Reactome: R-HSA-5083625
Reactome: R-HSA-5083632
Reactome: R-HSA-5083636
Reactome: R-HSA-5621480
Reactome: R-HSA-913709
Reactome: R-HSA-977068
Cellular Localisation Secreted
Alternative Antibody Names Anti-Mucin-5ac antibody
Anti-Muc-5ac antibody
Anti-Gastric Mucin antibody
Anti-Major Airway Glycoprotein antibody
Anti-Mucin-5 Subtype Ac - Tracheobronchial antibody
Anti-Tracheobronchial Mucin antibody
Anti-Tbm antibody
Anti-MUC5AC antibody
Anti-MUC5 antibody

Information sourced from Uniprot.org

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