• Western blot analysis of extracts of various cell lines, using MTIF3 antibody (STJ110822) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using MTIF3 antibody (STJ110822) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MTIF3 antibody (1-278) (STJ110822)

SKU:
STJ110822

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Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MTIF3 (1-278) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MTIF3
Gene ID: 219402
Uniprot ID: IF3M_HUMAN
Immunogen Region: 1-278
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-278 of human MTIF3 (NP_690876.3).
Immunogen Sequence: MAALFLKRLTLQTVKSENSC IRCFGKHILQKTAPAQLSPI ASAPRLSFLIHAKAFSTAED TQNEGKKTKKNKTAFSNVGR KISQRVIHLFDEKGNDLGNM HRANVIRLMDERDLRLVQRN TSTEPAEYQLMTGLQILQER QRLREMEKANPKTGPTLRKE LILSSNIGQHDLDTKTKQIQ QWIKKKHLVQITIKKGKNVD VSENEMEEIFHQILQTMPGI ATFSSRPQAVQGGKALMCV
Function IF-3 binds to the 28S ribosomal subunit and shifts the equilibrum between 55S ribosomes and their 39S and 28S subunits in favor of the free subunits, thus enhancing the availability of 28S subunits on which protein synthesis initiation begins.
Protein Name Translation Initiation Factor If-3 - Mitochondrial
If-3(Mt
If-3mt
If3(Mt
If3mt
Database Links Reactome: R-HSA-5368286
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Translation Initiation Factor If-3 - Mitochondrial antibody
Anti-If-3(Mt antibody
Anti-If-3mt antibody
Anti-If3(Mt antibody
Anti-If3mt antibody
Anti-MTIF3 antibody
Anti-DC38 antibody

Information sourced from Uniprot.org

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