• Western blot analysis of extracts of various cell lines, using MTHFD1L antibody (STJ110276) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using MTHFD1L Rabbit polyclonal antibody (STJ110276) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat brain using MTHFD1L rabbit polyclonal antibody (STJ110276) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using MTHFD1L Rabbit polyclonal antibody (STJ110276) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse testis using MTHFD1L rabbit polyclonal antibody (STJ110276) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using MTHFD1L Rabbit polyclonal antibody (STJ110276) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse spinal cord using MTHFD1L rabbit polyclonal antibody (STJ110276) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using MTHFD1L antibody (STJ110276) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MTHFD1L antibody (759-978) (STJ110276)

SKU:
STJ110276

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MTHFD1L (759-978) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MTHFD1L
Gene ID: 25902
Uniprot ID: C1TM_HUMAN
Immunogen Region: 759-978
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 759-978 of human MTHFD1L (NP_056255.2).
Immunogen Sequence: GVPLKKEYTEENIQLVADGC CNLQKQIQITQLFGVPVVVA LNVFKTDTRAEIDLVCELAK RAGAFDAVPCYHWSVGGKGS VDLARAVREAASKRSRFQFL YDVQVPIVDKIRTIAQAVYG AKDIELSPEAQAKIDRYTQQ GFGNLPICMAKTHLSLSHQP DKKGVPRDFILPISDVRASI GAGFIYPLVGTMSTMPGLPT RPCFYDIDLDTETEQVKGLF
Tissue Specificity Detected in most tissues, highest expression found in placenta, thymus and brain. Low expression is found in liver and skeletal muscle. Up-regulated in colon adenocarcinoma.
Function May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism complementing thus the enzymatic activities of MTHFD2.
Protein Name Monofunctional C1-Tetrahydrofolate Synthase - Mitochondrial
Formyltetrahydrofolate Synthetase
Database Links Reactome: R-HSA-196757
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Monofunctional C1-Tetrahydrofolate Synthase - Mitochondrial antibody
Anti-Formyltetrahydrofolate Synthetase antibody
Anti-MTHFD1L antibody
Anti-FTHFSDC1 antibody

Information sourced from Uniprot.org

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