![Immunofluorescence analysis of U-2 OS cells using [KO Validated] MTCH2 Rabbit polyclonal antibody (STJ114800) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/93887/375093/STJ114800_1__45731.1713141776.jpg?c=1 "Immunofluorescence analysis of U-2 OS cells using [KO Validated] MTCH2 Rabbit polyclonal antibody (STJ114800) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] MTCH2 Rabbit polyclonal antibody (STJ114800) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/93887/375094/STJ114800_2__68741.1713141777.jpg?c=1 "Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] MTCH2 Rabbit polyclonal antibody (STJ114800) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of lysates from wild type (WT) and MTCH2 knockout (KO) HeLa (KO) cells, using [KO Validated] MTCH2 Rabbit polyclonal antibody (STJ114800) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/93887/375095/STJ114800_3__50734.1713141778.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MTCH2 knockout (KO) HeLa (KO) cells, using [KO Validated] MTCH2 Rabbit polyclonal antibody (STJ114800) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunofluorescence analysis of U-2 OS cells using [KO Validated] MTCH2 Rabbit polyclonal antibody (STJ114800) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/93887/375093/STJ114800_1__45731.1713141776.jpg?c=1 "Immunofluorescence analysis of U-2 OS cells using [KO Validated] MTCH2 Rabbit polyclonal antibody (STJ114800) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
| Short Description : | Rabbit polyclonal MTCH2 (30-175) antibody for use in WB, IHC-P, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MTCH2 |
| Gene ID: | 23788 |
| Uniprot ID: | MTCH2_HUMAN |
| Immunogen Region: | 30-175 |
| Immunogen Sequence: | VGYEPLPPTIGRNIFGRQVC QLPGLFSYAQHIASIDGRRG LFTGLTPRLCSGVLGTVVHG KVLQHYQESDKGEELGPGNV QKEVSSSFDHVIKETTREMI ARSAATLITHPFHVITLRSM VQFIGRESKYCGLCDSIITI YREEGI |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-175 of human MTCH2 (NP_055157.1). |
| Function | Protein insertase that mediates insertion of transmembrane proteins into the mitochondrial outer membrane. Catalyzes insertion of proteins with alpha-helical transmembrane regions, such as signal-anchored, tail-anchored and multi-pass membrane proteins. Does not mediate insertion of beta-barrel transmembrane proteins. Also acts as a receptor for the truncated form of pro-apoptotic BH3-interacting domain death agonist (p15 BID) and has therefore a critical function in apoptosis. Regulates the quiescence/cycling of hematopoietic stem cells (HSCs). Acts as a regulator of mitochondrial fusion, essential for the naive-to-primed interconversion of embryonic stem cells (ESCs). Acts as a regulator of lipid homeostasis and has a regulatory role in adipocyte differentiation and biology. |
| Protein Name | Mitochondrial Carrier Homolog 2Met-Induced Mitochondrial Protein |
| Database Links | |
| Cellular Localisation | Mitochondrion Outer MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Mitochondrial Carrier Homolog 2 antibodyAnti-Met-Induced Mitochondrial Protein antibodyAnti-MTCH2 antibodyAnti-MIMP antibodyAnti-HSPC032 antibody |
Information sourced from Uniprot.org
