• Western blot analysis of extracts of various cell lines, using MTCH1 antibody (STJ110366) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using MTCH1 antibody (STJ110366) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human breast cancer using MTCH1 antibody (STJ110366) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using MTCH1 antibody (STJ110366) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-MTCH1 antibody (100-250) (STJ110366)

SKU:
STJ110366

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MTCH1 (100-250) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MTCH1
Gene ID: 23787
Uniprot ID: MTCH1_HUMAN
Immunogen Region: 100-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-250 of human MTCH1 (NP_001258570.1).
Immunogen Sequence: LYVKLLIQVGHEPMPPTLGT NVLGRKVLYLPSFFTYAKYI VQVDGKIGLFRGLSPRLMSN ALSTVTRGSMKKVFPPDEIE QVSNKDDMKTSLKKVVKETS YEMMMQCVSRMLAHPLHVIS MRCMVQFVGREAKYSGVLSS IGKIFKEEGLL
Tissue Specificity Widely expressed with a predominant expression in brain.
Function Protein insertase that mediates insertion of transmembrane proteins into the mitochondrial outer membrane. Catalyzes insertion of proteins with alpha-helical transmembrane regions, such as signal-anchored, tail-anchored and multi-pass membrane proteins. Does not mediate insertion of beta-barrel transmembrane proteins. May play a role in apoptosis.
Protein Name Mitochondrial Carrier Homolog 1
Presenilin-Associated Protein
Cellular Localisation Mitochondrion Outer Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Mitochondrial Carrier Homolog 1 antibody
Anti-Presenilin-Associated Protein antibody
Anti-MTCH1 antibody
Anti-PSAP antibody
Anti-CGI-64 antibody
Anti-UNQ1871 antibody
Anti-PRO4314 antibody

Information sourced from Uniprot.org

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