• Western blot analysis of extracts of various cell lines, using MT-ND5 antibody (STJ114339) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MT-ND5 antibody (300-400) (STJ114339)

SKU:
STJ114339

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MT-ND5 (300-400) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MT-ND5
Gene ID: 4540
Uniprot ID: NU5M_HUMAN
Immunogen Region: 300-400
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of mouse MT-ND5 (NP_904338.1).
Immunogen Sequence: KIIAFSTSSQLGLMMVTLGM NQPHLAFLHICTHAFFKAML FMCSGSIIHSLADEQDIRKM GNITKIMPFTSSCLVIGSLA LTGMPFLTGFYSKDLIIEAI N
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.
Protein Name Nadh-Ubiquinone Oxidoreductase Chain 5
Nadh Dehydrogenase Subunit 5
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Nadh-Ubiquinone Oxidoreductase Chain 5 antibody
Anti-Nadh Dehydrogenase Subunit 5 antibody
Anti-MT-ND5 antibody
Anti-MTND5 antibody
Anti-NADH5 antibody
Anti-ND5 antibody

Information sourced from Uniprot.org

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